Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue viruses

摘要: Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations rate, one case order, migration different viruses was detected system. However, dsRNA profiles eleven were found be identical when analysed by electrophoresis. Functionally that migrate relative positions this From these data we propose a modified version protein coding assignments published for 1 South Africa (Mertens et al., 1984) which identification would based upon order rather than The assignments, unlike original applicable all those appear likely valid normal isolates.