Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum

摘要: Abstract Rare nucleated fetal cells circulate within maternal blood. Noninvasive prenatal diagnosis by isolation and genetic analysis of these is currently being undertaken. We sought to determine if evidence existed for persistent circulation from prior pregnancies. Venous blood samples were obtained 32 pregnant women 8 nonpregnant who had given birth males 6 months 27 years earlier. Mononuclear sorted flow cytometry using antibodies CD antigens 3, 4, 5, 19, 23, 34, 38. DNA cells, amplified PCR Y chromosome sequences, was considered predictive a male fetus or cells. In the pregnancies, detected in 13 19 carrying fetus. 4 pregnancies with female fetuses, also detected. All pregnancies; 2 other terminations pregnancy. women, CD34+CD38+ even woman her last son sampling. Our data demonstrate continued CD34+ The prolonged persistence progenitor may represent human analogue microchimerism described mouse have significance development tolerance Pregnancy thus establish long-term, low-grade chimeric state female.