Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes.
作者： Markus Grompe , Milton Finegold , Muhsen Al-Dhalimy , Ken Overturf , Ching Nan Ou
DOI:
关键词: Transplantation 、 Stem cell 、 Serial Transplantation 、 Haematopoiesis 、 Pathology 、 Cell biology 、 Liver Stem Cell 、 Liver cytology 、 Liver regeneration 、 Biology 、 Fumarylacetoacetate hydrolase
摘要: Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation fumarylacetoacetate hydrolase deficiency murine model liver repopulation. Hepatocytes from donors were serially transplanted limiting numbers six and resulted complete repopulation during each cycle. This corresponds to a minimal number 69 cell doublings or 7.3 x 10(20)-fold expansion. No evidence for abnormal function altered hepatic architecture was found repopulated animals. We conclude fraction have growth potential similar hematopoietic stem cells.
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