L-Carnitine Production Through Biosensor-Guided Construction of the Neurospora crassa Biosynthesis Pathway in Escherichia coli.

摘要: L-Carnitine is a bioactive compound derived from L-lysine and S-adenosyl-L-methionine, which closely associated with the transport of long-chain fatty acids in intermediary metabolism eukaryotes sought after pharmaceutical, food, feed industries. The L-carnitine biosynthesis pathway has not been observed prokaryotes, use eukaryotic microorganisms as natural producers lacks economic viability due to complex cultivation low titers. While biotransformation processes based on petrochemical achiral precursors have described for bacterial hosts, fermentative de novo synthesis established although it holds potential sustainable economical one-pot process using renewable feedstocks. This study describes metabolic engineering Escherichia coli production. enzymes fungus Neurospora crassa that were functionally active E. identified applied individually or cascades assemble optimize four-step this host. Pathway performance was monitored by transcription factor-based biosensor. engineered strain produced supplemented L-Ne-trimethyllysine whole cell biotransformation, resulting 15.9 μM carnitine found supernatant. Notably, also 1.7 glycerol ammonium carbon nitrogen sources through endogenous Ne-trimethyllysine. work provides proof concept production coli, does depend precursors, but makes feedstocks instead. To best our knowledge, first description an bacterium.