Natural strategies for the spatial optimization of metabolism in synthetic biology.

作者: Christina M Agapakis , Patrick M Boyle , Pamela A Silver

DOI: 10.1038/NCHEMBIO.975

关键词: Metabolic pathwayMetabolic engineeringComputational biologyMetabolismDirected Molecular EvolutionSpatial optimizationSynthetic biologyFlux (metabolism)BiologyMetabolic regulation

摘要: Metabolism is a highly interconnected web of chemical reactions that power life. Though the stoichiometry metabolism well understood, multidimensional aspects metabolic regulation in time and space remain difficult to define, model engineer. Complex conversions can be performed by multiple species working cooperatively exchanging metabolites via structured networks organisms resources. Within cells, spatially regulated sequestration subcellular compartments through assembly multienzyme complexes. Metabolic engineering synthetic biology have had success first second dimensions, designing linear pathways channeling flux. More recently, third dimension has improved output engineered isolation organization multicell This review highlights natural examples three-dimensional both inter- intracellularly, offering tools perspectives for biological design.

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