5‐Hydroxytryptamine is biotransformed by CYP2C9, 2C19 and 2B6 to hydroxylamine, which is converted into nitric oxide
作者： Caroline Fradette , Nobuharu Yamaguchi , Patrick Du Souich
关键词: CYP1A2 、 Biochemistry 、 Chemistry 、 Cytochrome P450 、 Cytochrome P-450 CYP2B6 、 CYP2B6 、 Catalase 、 Nitric oxide 、 Oxidase test 、 Hydroxylamine
摘要: There is circumstantial evidence suggesting that 5-hydroxytryptamine (5-HT) could be biotransformed by enzymatic systems other than monoamino oxidase A, and the isoforms of cytochrome P450 may a source nitric oxide. This study aimed to assess whether contributes 5-HT biotransformation, provide metabolism generates oxide. Addition cultured hepatocytes yielded 5-hydroxyindol acetic acid, formation modulated enzyme inducers inhibitors. Recombinant human CYP2B6, 2C9 2C19 in but not CYP1A2, 2D6 or 3A4. Cultured with generated oxide, amount which was altered In presence 2C19, relaxed precontracted isolated aortic rings, without endothelium, an effect prevented addition methylene blue inhibitor catalase, myoglobin. absence hydroxylamine always assayed as byproduct metabolism. In conclusion, biotransform 5-HT, yielding hydroxylamine, converted oxide catalase. British Journal Pharmacology (2004) 141, 407–414. doi:10.1038/sj.bjp.0705632
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