Tissue engineering potential of human dermis-isolated adult stem cells from multiple anatomical locations

摘要: Abundance and accessibility render skin-derived stem cells an attractive cell source for tissue engineering applications. Toward assessing their utility, the variability of constructs engineered from human dermis-isolated adult (hDIAS) was examined with respect to different anatomical locations (foreskin, breast, abdominal skin), both in vitro a subcutaneous, athymic mouse model. All yielded hDIAS multi-lineage differentiation potentials, though adipogenesis not seen foreskin-derived cells. Using cartilage as model, were compared. Construct morphology differed by location. The mechanical properties foreskin- similar at implantation, remaining comparable after 4 additional weeks culture vivo. Breast mechanically testable. For all groups, no signs abnormality observed host. Addition aggregate redifferentiation prior construct formation improved chondrogenic cells, evident increases glycosaminoglycan collagen contents. More robust Alcian blue staining homogeneous populations also compared controls. Human DIAS elicited adverse host responses, reacted positively regimens, possessed potential caveat that efficacy may differ origin skin. Taken together, these results suggest hold promise number