Alterations of FSH-stimulated progesterone production and calcium homeostasis in primarily cultured human luteinizing-granulosa cells induced by fenvalerate.
作者： Jun He , Jianfeng Chen , Ru Liu , Shoulin Wang , Lin Song
DOI: 10.1016/J.TOX.2004.05.014
关键词: Calcium 、 Endocrinology 、 Fenvalerate 、 Gonadotropin 、 Ovarian follicle 、 Granulosa cell 、 Calcium metabolism 、 Follicle-stimulating hormone 、 Biology 、 Trifluoperazine 、 Internal medicine
摘要: Fenvalerate, a synthetic pyrethroid, is widely used in agriculture and other domestic applications China. Recently, Fenvalerate has been suspected to be one of the endocrine-disrupting chemicals (EDC). In this study, we investigated effects fenvalerate on follicle-stimulating hormone (FSH)-stimulated progesterone (P4) production by human ovarian luteinizing-granulosa cells (hGLCs). After 24 h incubation, inhibited FSH-stimulated P4 production. At same time, cAMP also decreased. Due calcium Ca2+ -calmodulin (CaM) system involving gonadotropin-stimulated steroidogenesis granulosa cells, then evaluated trifluoperazine (TFP)- verapamil-driven The results showed that or calmodulin might play role fenvalerate-induced alterations biosynthesis. Then, homeostasis hGLCs were studied. result 5 microM induced slow increase [Ca2+]i using fluorescent indicator fluo-3/AM. changes total concentration CaM method immunofluorescence. There significant all treated groups. summary, could inhibit Also, interferes with hGLCs. may mediated partly through signal.
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