Degradation of 13C-labeled pyrene in soil-compost mixtures and fertilized soil

摘要: Polycyclic aromatic hydrocarbons (PAH) are toxic pollutants widely distributed in the environment due to natural and anthropogenic processes. In order mitigate tar oil contaminations with PAH, research on improving bioremediation approaches, which sometimes inefficient, is needed. However, knowledge fate of PAH-derived carbon microbial degraders particular compost-supplemented soils still limited. Here we show PAH turnover mass balance microcosms soil-compost mixtures or farmyard fertilized soil using [(13)C6]-pyrene as a model PAH. Complete pyrene degradation 100 mg/kg was observed all supplemented within 3 5 months, residual (13)C mainly found converted biomass. Long-term fertilization manure resulted removal efficiency similar compost addition, although much longer lag phase, higher mineralization, lower incorporation into Organic amendments either long-term amendment thus play key role increasing PAH-degrading potential community. Phospholipid fatty acid stable isotope probing (PLFA-SIP) used trace population amount biomass formed from degradation. The results demonstrate that complex degrader consortia rather than expected single players responsible for organic-amended soil.