17β-Hydroxysteroid Dehydrogenase from Human Red Blood Cells
作者： G M Jacobsohn , R B Hochberg
DOI: 10.1016/S0021-9258(18)93369-7
关键词: Chromatography 、 Sodium 、 Chemistry 、 Hydroxysteroid dehydrogenase 、 Dehydrogenase 、 Sulfate 、 Estradiol sulfate 、 Enzyme assay 、 Sephadex 、 Estrone
摘要: Abstract 17β-Hydroxysteroid dehydrogenase has been isolated from human red cells and purified 740 times. The enzyme is sensitive to ionic strength most effective at sodium chloride concentrations of 0.1 m above. 17β-Estradiol 3-sulfate reacts a faster rate than 17β-estradiol. Km for estradiol 9.1 x 10-5, sulfate it 3.8 10-4. free compound pH optimum 9.7; the optimally 9.0. specific TPN; DPN shows one-sixth one-tenth as much activity. values TPN are 2.7 10-5 with 2.4 sulfate. Acetyl-TPN less active TPN. Albumin inhibits reaction, presumably by binding steroids. Organic solvents also act inhibitorily. appears be sulfhydryl type. Enzyme activity was assayed measurement TPNH produced yield estrone. Reduction estrone or its occurs slower rates corresponding reverse reactions. molecular weight enzyme, calculated mobility data on Sephadex gel, approximately 75,500.
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