Lactobacillus brevis OK56 ameliorates high-fat diet-induced obesity in mice by inhibiting NF-κB activation and gut microbial LPS production

摘要: The anti-obesity effects of anti-inflammatory probiotics, isolated Lactobacillus brevis OK56 from the kimchi lactic acid bacteria collection, were examined and its on high-fat diet (HFD)-induced obesity (DIO) mice evaluated. strongly inhibited NF-κB activation TNF-α, IL-1β, IL-6 expression in lipopolysaccharide (LPS)-stimulated macrophages. Oral administration (1 × 109 CFU/mouse) suppressed HFD-induced increase body epididymal fat weight gains (by 28 31%, respectively), as well plasmatic atherogenic index, LPS level. Real-time PCR analysis revealed that also F4/80, CD68, CD204, IL-1β adipose tissue DIO mice. abrogated disturbance to gut microbiota, such reduction number bifidobacteria, colonic fluid LPS, breath H2 levels. inducible NO synthetase (iNOS) cyclooxygenase (COX)-2 nuclear factor (NF)-κB colon. Furthermore, HFD-reduced tight junction proteins ZO-1, occludin claudin-1. These results suggest has anti-obesity, which exerts through inhibition microbiota production, signaling pathway, macrophage infiltration into tissue.