Identification of latent neosporosis in sheep in Tehran, Iran by polymerase chain reaction using primers specific for the Nc-5 gene
作者： Mohsen Arbabi , Amir Abdoli , Abdolhossein Dalimi , Majid Pirestani
关键词: genomic DNA 、 Biology 、 Ovis 、 Neospora caninum 、 Polymerase chain reaction 、 Virology 、 Nested polymerase chain reaction 、 Parasite hosting 、 GenBank 、 Gene
摘要: Little is known about latent infection and molecular characterisation of Neospora caninum in sheep ( Ovis aries ). In this study, 330 samples (180 hearts 150 brains) were analysed for N. DNA by nested polymerase chain reaction (PCR) targeting the Nc-5 gene. was detected 3.9% (13/330) samples. The parasite’s 6.7% heart (12/180) 0.7% (1/150) brain No clinical signs recorded from infected or uninfected animals. Sequencing genomic revealed 96% – 99% similarity with each other 95.15% 100% sequences deposited GenBank. To our knowledge, first report on use PCR to identify neosporosis Iran. results study have potential contribute understanding role -infected epidemiology neosporosis.
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