Preparation of High‐Performance Water‐Soluble Quantum Dots for Biorecognition through Fluorescence Resonance Energy Transfer

作者: Chin-Ping Huang , Cheng-Fu Chao , Mo-Yuan Shen , Teng-Ming Chen , Yaw-Kuen Li

DOI: 10.1002/ASIA.201200526

关键词: Quantum yieldAdsorptionChemistryLuminescencePhotochemistrySuccinic acidFörster resonance energy transferQuantum dotMolecular recognitionAnalytical chemistryWater soluble

摘要: An improved method for the synthesis of high-performance and water-soluble quantum dots (QDs) in- volving encapsulation mercapto- succinic acid coated QDs (MSA-QDs) with poly(diallyldimethylammonium chloride) (PDDA) followed by their direct photoactivation fluorescent radiation near 295 K to yield PDDA- (PDDA-QDs) has been demonstrated. The (QY) PDDA-QDs was signifi- cantly from 0.6 (QY MSA-QDs) 48 %. By using this syn- thetic strategy, highly photolumines- cent varied size were readily prepared. surface proper- ties MSA-QDs extensively characterized. luminescent positively charged serve as a useful convenient tool protein adsorp- tion. With D 5 -3-ketosteroid isomerase adsorbed PDDA-QD complex, bio- recognition steroids demonstrat- ed through application fluores- resonance energy transfer.

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