Readily available porcine aortic valve matrices for use in tissue valve engineering. Is cryopreservation an option?

摘要: The clinical use of acellular biological valves as scaffolds in tissue valve engineering would require them to be readily available. This study examines the feasibility cryopreserving porcine aortic matrices for engineering. Matrices prepared using an enzymatic-detergent decellularization protocol were examined before and after cryopreservation. biochemical status tissues evaluated by collagen uronic acid (proteoglycan) determination their mechanical properties determined a burst test. histological ultrastructural light electron microscopy. Cryopreservation did not significantly affect content leaflet matrices. Histological sections, however, confirmed extensive disruption extracellular matrix inter-fibrillar proteoglycan associations following Although neither breakage force nor maximum at failure was different cryopreservation, strain observed higher To our knowledge this is first investigate effects cryopreservation on alter Nevertheless, had significant adverse structural Cryopreserved showed when stressed compared non cryopreserved While, theoretically are only expected functional limited time until regenerated vivo, further testing necessary evaluate these changes durability