The effects of elongational stress exposure on the activation and aggregation of blood platelets.

摘要: Hemodynamic shear is known to stimulate blood and endothelial cells induce platelet activation. Many studies of shear-induced stimulation have employed rotational viscometers in which secondary flow effects are assumed be negligible. Shear induced activation occurs at elevated rates where flows may contribute a significant percentage the total hydrodynamic force experienced by sample. Elongational stress, one component this flow, has been shown alter transmembrane ion flux intact cell permeability synthetic membrane preparations. also vasculature sites stress. Secondary components during stress application viscometry. A unique 'constrained convergence' elongational chamber was designed fabricated study response exposure. The capable producing an elongation rate 2.1 s-1 with corresponding volume averaged 58.33 s-1. Significant changes were observed distribution measured added chemical antagonists after histogram shifted toward larger particle sizes, suggesting formation large aggregates as result Platelets exposed demonstrated dose dependent decrease ADP-induced aggregation extent aggregation.