Genetic diversity and phylogeny of indigenous rhizobia nodulating faba bean (Vicia faba L.) in Greece.

摘要: Abstract The genetic diversity and phylogeny of fast-growing rhizobia isolated from root nodules Vicia faba grown in different geographical regions Greece were assessed. Although Rhizobium leguminosarum sv. viciae is the most common symbiont spp. European soils, there no available information on native nodulating bean Greece. Seventy bacterial strains grouped into sixteen distinct profiles based BOX-PCR fingerprinting. phylogenetic affiliation was further defined by sequence analysis rrs multilocus (MLSA) three housekeeping genes (recA, atpD gyrB). Fifty-eight isolates affiliated with recently described genospecies gsF-2, represented R. laguerreae FB206T, whereas six closely related to gsB two might belong gsA. Two assigned hidalgonense another non-nodulating could not be any validly species possibly a new rhizobial lineage. Interestingly, commonly found at all sampling sites, suggesting that they main symbionts beans Greek soils. According phylogenies symbiosis-related (nodC nifH), belonged symbiovar (sv.) harboring four nodC gene haplotypes clades together other countries continents. This first report viciae. No correlation observed between haplotypes, geographic origin chromosomal background study.