作者: Natasa Skoko , Marco Baralle , Sergio Tisminetzky , Emanuele Buratti
DOI: 10.1007/S12033-011-9390-X
关键词: Gene 、 Exon 、 Alternative splicing 、 RNA splicing 、 Biotechnology 、 Spliceosome 、 Post-transcriptional regulation 、 Intron 、 Biology 、 Regulation of gene expression
摘要: Eukaryotic gene expression relies on several complex molecular machineries that act in a highly coordinated fashion. These govern all the different steps of mRNA maturation, from transcription and pre-mRNA processing nucleus to export cytoplasm its translation. In particular, splicing process consists joining together sequences (known as “exons”) have be differentiated their intervening commonly referred “introns.” The required perform this is very dynamic macromolecular ribonucleoprotein assembly functions an enzyme, called “spliceosome.” Because flexibility, represents one main mechanisms qualitative quantitative regulation eukaryotic genomes. This flexibility mainly due possibility alternatively recognizing various exons are present molecule therefore enabling obtaining multiple transcripts same gene. However, by spliceosome also achieved through ability influence many other include transcription, export, stability, even protein Therefore, biotechnological point view can exploited improve production strategies processes molecules interest. work, we aimed provide overview how biotechnology applications may benefit introduction introns within sequence