A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA
作者： Wojciech Rychlik , Robert E. Rhoads
关键词: Biology 、 Combinatorial chemistry 、 Hybridization probe 、 Nucleic acid sequence 、 Multiplex ligation-dependent probe amplification 、 Nucleic acid 、 Molecular probe 、 Biochemistry 、 Nucleic acid thermodynamics 、 Oligonucleotide 、 Duplex (building)
摘要: A method is presented for choosing optimal oligodeoxyribonucleotides as probes filter hybridization, primers sequencing, or DNA amplification. Three main factors that determine the quality of a probe are considered: stability duplex formed between and target nucleic acid, specificity intended sequence, self-complementarity. calculations based on nearest-neighbor thermodynamic values determined by Breslauer et al. [Proc. Natl. Acad. Sci. U.S.A. (1986), 83: 3746]. Temperatures dissociation predicted described here were within 0.4 degrees C obtained experimentally ten oligonucleotides. Calculations its self-complementarity simple dynamic algorithm.
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