Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes
作者： Yinghui Li , Fengna Li , Binbin Lin , Xiangfeng Kong , Yulong Tang
DOI: 10.1007/S11033-014-3646-Z
关键词: Internal medicine 、 Endocrinology 、 Myogenin 、 Skeletal muscle 、 Interleukin 15 、 Myocyte 、 Cell type 、 Cellular differentiation 、 Biology 、 Cell biology 、 Myokine 、 Desmin
摘要: The present study was carried out to preliminarily reveal the underlying mechanisms of co-culture system between porcine muscle satellite cells (SCs) and stromal-vascular (SVs). two cell types were co-cultured assess both proliferation differentiation. Desmin Pref-1 immunofluorescence staining technique taken identify isolated cells. expression specific marker genes Myogenin up-regulated in SCs (P < 0.05) differentiation could be promoted when with preadipocytes compared single-cultured control, while c/EBPβ SVs down-regulated inhibited. Furthermore, secretion myokine IL-15 markedly increased, as well its gene protein levels supernatants. However, adipokine leptin significantly decreased. These findings demonstrate that myokines like facilitate SCs' inhibit differentiation, act an important regulator adipocytes.
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