Application of BIOMED-2 clonality assays to formalin-fixed paraffin embedded follicular lymphoma specimens: Superior performance of the IGK assays compared to IGH for suboptimal specimens
作者： Anna Margrét Halldórsdóttir , Barbara A. Zehnbauer , W. Richard Burack
DOI: 10.1080/10428190701377022
关键词: Follicular lymphoma 、 Lymphoma 、 Immunoglobulin Gene Rearrangement 、 Pathology 、 Molecular biology 、 Molecular genetics 、 Gene rearrangement 、 DNA extraction 、 Polymerase chain reaction 、 Biology 、 Chronic lymphocytic leukemia 、 Cancer research 、 Oncology 、 Hematology
摘要: The BIOMED-2 PCR-based immunoglobulin gene rearrangement assays have quickly become the most commonly used laboratory method for detection of B-cell clonality. Therefore, reliability these under various conditions has increasingly important. When studying paired cases follicular lymphoma (FL) from individual patients, we to assess clonality in 40 formalin-fixed paraffin-embedded (FFPE) specimens 19 patients diagnosed with FL. IGH failed give a clonal result 26/40 (65%) specimens, while IGK only 3/40 (8%) specimens. high failure rate this set FFPE lymphomas cannot be explained by systematic problems DNA extraction or amplification because same resulted low (3/32, 9%) small lymphocytic lymphoma/chronic leukemia and fresh frozen FL (1/6, 17%). Furthermore, second validation 13 was 9/13 (69%). assay did not perform well on may superior assessing when no fresh/frozen tissue is available.
informahealthcare.com参考文章(27)
Jerry Z. Gong, Sherman Zheng, Roberto Chiarle, Christine De Wolf-Peeters, Giorgio Palestro, Glauco Frizzera, Giorgio Inghirami, Detection of Immunoglobulin κ Light Chain Rearrangements by Polymerase Chain Reaction: An Improved Method for Detecting Clonal B-Cell Lymphoproliferative Disorders American Journal of Pathology. ,vol. 155, pp. 355- 363 ,(1999) , 10.1016/S0002-9440(10)65132-2
Inghirami G, Knowles Dm, Szabolcs Mj, Corradini P, Cesarman E, Yee Ht, Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction. Laboratory Investigation. ,vol. 68, pp. 746- 757 ,(1993)
B G Van Ness, D J Martin, R M Feddersen, The frequency of multiple recombination events occurring at the human Ig kappa L chain locus. Journal of Immunology. ,vol. 144, pp. 1088- 1093 ,(1990)
Adam Bagg, Rita M. Braziel, Daniel A. Arber, Karen E. Bijwaard, Albert Y. Chu, Immunoglobulin Heavy Chain Gene Analysis in Lymphomas: A Multi-Center Study Demonstrating the Heterogeneity of Performance of Polymerase Chain Reaction Assays The Journal of Molecular Diagnostics. ,vol. 4, pp. 81- 89 ,(2002) , 10.1016/S1525-1578(10)60685-X
Reetesh K. Pai, Artemis E. Chakerian, John M. Binder, Mitual Amin, David S. Viswanatha, B-Cell Clonality Determination Using an Immunoglobulin κ Light Chain Polymerase Chain Reaction Method The Journal of Molecular Diagnostics. ,vol. 7, pp. 300- 307 ,(2005) , 10.1016/S1525-1578(10)60558-2
J.H.J.M. Van Krieken, A.W. Langerak, J.F. San Miguel, A. Parreira, J.L. Smith, G.M. Morgan, M. Kneba, E.A. MacIntyre, J.J.M. Van Dongen, Clonality analysis for antigen receptor genes: Preliminary results from the biomed-2 concerted action PL 96-3936 Human Pathology. ,vol. 34, pp. 359- 361 ,(2003) , 10.1053/HUPA.2003.99
L. JUUL, L. HOUGS, V. ANDERSEN, A. SVEJGAARD, T. BARINGTON, The normally expressed kappa immunoglobulin light chain gene repertoire and somatic mutations studied by single-sided specific polymerase chain reaction (PCR); frequent occurrence of features often assigned to autoimmunity. Clinical and Experimental Immunology. ,vol. 109, pp. 194- 203 ,(1997) , 10.1046/J.1365-2249.1997.4341332.X
TC Diss, HX Liu, MQ Du, PG Isaacson, None, Improvements to B cell clonality analysis using PCR amplification of immunoglobulin light chain genes Molecular Pathology , 55 (2) pp. 98-101. (2002). ,vol. 55, pp. 98- 101 ,(2002) , 10.1136/MP.55.2.98
Alberto Achille, Aldo Scarpa, Marina Montresor, Maria Scardoni, Giuseppe Zamboni, Marco Chilosi, Paola Capelli, Giuseppe Franzin, Fabio Menestrina, Routine application of polymerase chain reaction in the diagnosis of monoclonality of B-cell lymphoid proliferations. Diagnostic Molecular Pathology. ,vol. 4, pp. 14- 24 ,(1995) , 10.1097/00019606-199503000-00005
J J M van Dongen, A W Langerak, M Brüggemann, P A S Evans, M Hummel, F L Lavender, E Delabesse, F Davi, E Schuuring, R García-Sanz, J H J M van Krieken, J Droese, D González, C Bastard, H E White, M Spaargaren, M González, A Parreira, J L Smith, G J Morgan, M Kneba, E A Macintyre, Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. ,vol. 17, pp. 2257- 2317 ,(2003) , 10.1038/SJ.LEU.2403202