Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells

摘要: Lung cancer has long been the most dangerous malignant tumor among males in both well developed and poorly countries. Radiotherapy plays a critical role curative management of inoperable non-small cell lung (NSCLC) is also used as post-surgical treatment patients. Radioresistance an important factor that limits efficacy radiotherapy for NSCLC Increasing evidence suggests microRNAs (miRNAs) possess diverse cellular regulatory roles radiation responses. In this study, we miRNA microarray technology to identify serum miRNAs were differentially expressed before after We further examined biological function miR-208a on viability, apoptotic death cycle distribution human cells explored probable mechanism. Nine miRNAs, including miR-29b-3p, miR-200a-3p, miR-126-3p significantly down-regulated, whereas was only up-regulated patients (P < 0.05). The expression could be induced by X-ray irradiation cells. Forced promoted proliferation radioresistance via targeting p21 with corresponding activation AKT/mTOR pathway cells, down-regulation resulted opposite effects. addition, increased percentage undergoing apoptosis inhibited G1 phase arrest Moreover, from exosome fraction shuttle A549 time-dependent manner, which likely contribute subsequent present study provides can affect radiosensitivity transported exosomes. Thus, may serve potential therapeutic target