Uncouplers can shuttle between localized energy-coupling sites during photophosphorylation by chromatophores of Rhodopseudomonas capsulata N22

摘要: Two models of the action uncoupler molecules in inhibiting photophosphorylation bacterial chromatophores are considered: either shuttle rapidly between energy-coupling sites, or that bound to particular sites for a time is comparable with turnover apparatus may uncouple by co-operative "substoichiometric' mechanism. It found titre necessary cause complete uncoupling lowered if rate initially decreased partially restricting electron flow an appropriate antimycin A. This result indicates energy coupling which energized intermediate transport and phosphorylation delocalized over entire chromatophore membrane those it not. If restricted covalent H+-translocating ATP synthase inhibitor dicyclohexylcarbodi-imide, effect inhibition also relative H+-ATP absent. important appears be inconsistent electron-transport "energized state' set up utilized membrane.