Homer expression in the Xenopus tadpole nervous system.

摘要: Homer proteins are integral components of the postsynaptic density and thought to function in synaptogenesis plasticity. In addition, overexpression developing Xenopus retinotectal system results axonal pathfinding errors. Here we report that contains homer1 gene, expressed as isoform, xhomer1b, which is highly homologous mammalian homer1b. The gene three isoforms, truncated or short form homer1a long forms homer1b -1c. For Xenopus, cloned very similar variants homer1b, identified xhomer1b.1, xhomer1b.2, xhomer1b.3, display up 98% homology with each other 90% similarity Furthermore, demonstrate also a Homer1 protein, could be induced by kainic acid injection likely Homer1a. xHomer1b expression was unaffected neuronal activity levels but developmentally regulated. Within brain, spatial temporal distributions both isoforms were neuropil cell body regions. detected motor axons. Differential distribution two apparent: Homer1b immunoreactivity prominent at junctions between soma ventricular surface; retina, Mueller radial glia immunoreactive for Homer1, not Homer1b, suggesting retinal contain only Homer1a isoform. muscle throughout development aligned actin striations skeletal muscle. high level conservation xhomer1 protein nervous suggest may important normal circuit development.