Methylxanthine-evoked perturbation of spontaneous and evoked activities in isolated newborn rat hippocampal networks.

摘要: Treatment of apnea prematurity with methylxanthines like caffeine, aminophylline or theophylline can evoke hippocampal seizures. However, it is unknown at which interstitial brain concentrations promote such neonatal seizures interfere physiological 'early network oscillations' (ENOs) that are considered as pivotal for maturation neural networks. We studied and caffeine effects on ENOs in CA3 neurons (CA3-ENOs) electrical stimulation-evoked monosynaptic CA1 field potentials (CA1-FPs) sliced intact hippocampi, respectively, from 8 to 10-days-old rats. Submillimolar doses blocking adenosine receptors phosphodiesterase-4 (PDE4), did not affect CA3-ENOs, ENO-associated cytosolic Ca(2+) transients CA1-FPs nor they provoke seizure-like discharges. Low millimolar (⩾1mM) (⩾5mM), GABAA glycine plus sarcoplasmic-endoplasmic reticulum ATPase (SERCA)-type ATPases, evoked discharges no indication dysregulation. Inhibiting PDE4 rolipram strychnine had effect CA3-ENOs occlude events tested theophylline. receptor blockade induced occluded theophylline-evoked the slices, but hippocampi. In summary, submillimolar methylxanthine do acutely spontaneous electrically synaptic activities low needed isolated developing conclude mechanisms methylxanthine-related involve SERCA inhibition-related neuronal dysregulation, inhibition, whereas GABA(A) may contribute partially.