Human Phenol Sulfotransferase STP2 Gene: Molecular Cloning, Structural Characterization, and Chromosomal Localization

摘要: Sulfonation is an important pathway in the biotransformation of many drugs, xenobiotics, neurotransmitters, and steroid hormones. The thermostable (TS) form phenol sulfotransferase (PST) preferentially catalyzes sulfonation {open_quotes}simple{close_quotes} planar phenols, levels activity TS PST human tissues are controlled by inheritance. Two different liver cDNAs have been cloned that encode proteins with amino acid sequences 96% identical. We determined structure chromosomal localization gene for one these two cDNAs, STP2, as a step toward understanding molecular genetic mechanisms involved regulation this enzyme humans. STP2 spans approximately 5.1 kb contains nine exons range length from 74 to 347 bp. locations most exon-intron splice junctions identical those thermolabile humans, STM; rat gene; estrogen ST (EST) gene, STE; guinea pig EST gene. initial exons, IA IB, were identified performing 5{prime}-rapid amplification cDNA ends template. Exons IB noncoding represent cDNAmore » 5{prime}untranslated region sequences. apparent 5{prime}-ons contain no canonical TATA boxes, but do CCAAT elements. was localized chromosome 16 PCR DNA NIGMS human/rodent somatic cell hybrids Structural characterization will make it possible begin study tissues. 63 refs., 7 figs., 1 tab.« less