A novel low-molecular weight alkaline mannanase from Streptomyces tendae
作者： Hah Young Yoo , G. C. Pradeep , Seung Wook Kim , Don Hee Park , Yun Hee Choi
DOI: 10.1007/S12257-014-0885-8
关键词: Chromatography 、 Mannobiose 、 Thin-layer chromatography 、 Industrial and production engineering 、 Chemistry 、 Hydrolysis 、 Metal ions in aqueous solution 、 Oligosaccharide 、 Molecular mass 、 Sepharose
摘要: A novel, low-molecular weight, alkaline mannanase from Streptomyces tendae (MnSt) was purified to homogeneity and biochemically characterized. The extracellular with 26.3% yield using a Sepharose Cl-6B column. molecular mass of MnSt approximately 24 kDa. stable over broad pH range (5 ∼ 12.5), thermally at 60°C, functioned optimally 50°C 12.0. had Km Vmax values 0.05 ± 1 mg/mL 439 0.5mmol/min, respectively, bean gum galactomannan as substrate. N-terminal sequence GWSVDAPYIAXQPFS. Thin layer chromatography (TLC) analysis the hydrolysis products suggested that major oligosaccharide produced mannobiose. activity remarkably affected by metal ions, modulators, chelators, detergents. simple purify, high thermal stability, range, mannooligosaccharides. has potential for use an industrial biocatalyst, particularly bio-bleaching agent or production.
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