Digital imaging fluorescence microscopy

摘要: Publisher Summary This chapter discusses the unique features of digital imaging microscopy and suggests several research areas in lipoprotein metabolism where technology can be applied to great advantage. The relationship between plasma lipoproteins atherosclerosis is elusive because complexity interactions different cell types normal blood vessel wall. Action lysosomal enzymes on low-density (LDL) necessary for subsequent regulatory processes. As protein moiety must degraded, regulation involves lipid components. Although lipid-protein studied directly test tube using spectroscopic techniques, similar studies role lipids situ are technically impossible conventional biochemical methods. One strategy combine use fluorescent analogs with fluorescence microscopy. best approach apply existing hardware software digitize images living cells exposed a particular lipid. ability make quantitative measurements depends resolution three major experimental problems: (1) elimination photobleaching, (2) calibration standards, (3) out-of-plane contributions intensities.