Target sites for SINE integration in Brassica genomes display nuclear matrix binding activity.

摘要: Short interspersed nuclear elements (SINEs) are ubiquitous components of complex animal and plant genomes. SINEs believed to be important players in eukaryotic genome evolution. Studies on SINE integration sites have revealed non-random without strict nucleotide sequence requirements for the target, suggesting that targeted DNA might assume specific secondary structures or protein associations. Here, we report S1 genomes Brassica show an interesting preference matrix attachment regions (MARs). Ten cloned genomic were tested their ability bind both before after a event. Eight by display strong affinity matrix, while two weaker binding. The did not any matrix-binding capacity its own either non-methylated methylated forms. In vivo, integrated is surrounding may remain undermethylated undergo methylation. However, containing methylated'S1, with flanking sequences, found vary vitro. These results suggest possible molecular basis preferential targeting MARs impact events upon gene function.