Self-healing functionalization of sulfonated hafnium oxide and copper oxide nanocomposite for effective biocidal control of multidrug-resistant bacteria

摘要: The intensive use of antibiotics in hospitals, poultry, and other industries has led to the emergence bacteria resistant most commercially available antibiotics. These antibiotic-resistant (ARB) are being released into aquatic environment could cause serious health issues. In this study, multidrug-resistant (MDR) were isolated from effluent secondary process (activated sludge) full-scale hospital wastewater treatment plants (HWWTPs). bacterial isolates tested against explore their resistance profile. Sulfonated hafnium oxide-doped CuO nanoparticles (Cu/SHfNP) synthesized improve antibacterial antibiofilm activities Gram-negative (Enterobacter asburiae, Escherichia coli, Pseudomonas aeruginosa PA01) Gram-positive (Staphylococcus aureus ATCC6538) strains. combined effects SHfNP at 0.5 mM 1 exhibited strong MDR bacteria. results showed that Cu/SHfNP was an effective alternative agent inhibiting persister cells, planktonic mixed-species biofilm formation on polystyrene, nylon, glass surfaces. Confocal laser scanning microscopy (CLSM) electron (SEM) thickness cell attachment reduced significantly when exposed Cu/SHfNP. photocatalytic activity revealed maximum reduction growth by 6.1 log CFU mL−1 under visible light irradiation for 180 min. nontoxic human meaning it can be used as a self-healing combat prevent membrane biofouling membrane-based bioreactors.