Quantitation of DNA methylation in Epstein-Barr virus–associated nasopharyngeal carcinoma by bisulfite amplicon sequencing

摘要: Epigenetic changes, including DNA methylation, disrupt normal cell function, thus contributing to multiple steps of carcinogenesis. Nasopharyngeal carcinoma (NPC) is endemic in southern China and highly associated with Epstein-Barr virus (EBV) infection. Significant changes the host methylome are observed EBV-associated NPC cancer development. marks for diagnosis urgently needed. In order explore methylation marks, we investigated candidate genes nasopharyngeal carcinoma. We first employed methyl-capture sequencing cDNA microarrays compare genome-wide profiles seven tissues five non-cancer epithelium (NNE) tissues. found 150 hypermethylated CpG islands spanning promoter regions down-regulated genes. Furthermore, quantified rates using bisulfite amplicon nine NNE All showed significantly higher than tissues, ratios (NPC/NNE) were descending as follows: ITGA4 > RERG > ZNF671 > SHISA3 > ZNF549 > CR2 > RRAD. particular, levels ITGA4, RERG, ZNF671 could distinguish patients from subjects. identified previously unidentified The combination targeted profiling by next-generation sequencers should provide useful information regarding cancer-specific aberrant methylation.