Regulation and release of VSH-1, a prophage and gene transfer agent of Brachyspira hyodysenteriae

摘要: Brachyspira hyodysenteriae is an anaerobic spirochete that harbors the mitomycin Cinducible prophage, VSH-1. VSH-1 defective in replication and viral packaging. Instead of a genome, virions package random 7.5-kb fragments B. chromosomal DNA mediate gene transfer between cells. structural genes were identified based on matches to N-terminal amino acid sequences determined for several head tail-associated proteins. The sequenced mapped 14.3-kb region chromosome. sequence was delimited upstream downstream by open reading frames with high degree translated homology bacterial genes. closely grouped morphogenetic preceding tail An adjacent frame bacteriophage endolysins identified. arrangement consistent organization late operons.