Measurement of fluid viscosity at microliter volumes using quartz impedance analysis

摘要: The purpose of this work was to measure viscosity fluids at low microliter volumes by means quartz crystal impedance analysis. To achieve this, a novel setup designed that allowed for measurement 8 10 μL. technique based on the principle electromechanical coupling piezoelectric crystals. arrangement simple with times ranging from 2 3 minutes. assembly did not impose any unwanted initial stress unloaded crystal. Quartz crystals 5- and 10-MHz fundamental frequency were calibrated glycerol-water mixtures known density prior measurements. True shifts, work, determined followed aqueous solutions sucrose, urea, PEG-400, glucose, ethylene glycol 25°C±0.5°C. measured viscosities found be reproducible consistent values reported in literature. Minor inconsistencies resistance shifts affect results significantly, experimental origin rather than due electrode surface roughness. Besides, as expected viscoelastic fluid, PEG 8000 solutions, calculated less dependence storage loss modulus components complex viscosity. From results, it can concluded present provide accurate assessment Newtonian also shows potential analyzing non-Newtonian volumes.