Nucleic acid sequence amplification methods utilizing a transcription complex
作者： Daniel Louis Kacian , Timothy J. Fultz
DOI:
关键词: Computational biology 、 Nucleic acid sequence 、 Transcription preinitiation complex 、 Cloning 、 RNA 、 Sequence (medicine) 、 Biology 、 Molecular biology 、 Nucleic acid 、 Nucleic acid methods
摘要: Methods of synthesizing multiple copies a target nucleic acid sequence autocatalytically under conditions substantially constant temperature, ionic strength, and pH are provided in which RNA the generate additional copies. These methods useful for generating purposes include assays to quantitate specific sequences clinical, environmental, forensic similar samples, cloning probes.
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