Evaluation of In Vitro Assays For Assessing the Toxicity of Cigarette Smoke and Smokeless Tobacco

摘要: Background: In vitro toxicology studies of tobacco and smoke have been used to understand why use causes cancer assess the toxicologic impact product design changes. The need for has heightened given Food Drug Administration's newly granted authority over products mandating performance standards evaluate manufacturers' health claims about modified products. goal this review is critically in methods related assessing identify research gaps. Methods: PubMed database searches were tobacco-related published since 1980. Articles before 1980 with high relevance also identified. data compiled examine ( a ) goals study, b collecting test substances, c experimental designs, d end points, e risk. Results: A variety assays are available that address different modes action, mostly using non–human cell models. However, smokeless perform poorly these assays. Although reliable as screening tool qualitative assessments, validated quantitative comparisons Assay batteries not developed, although they exist nontobacco assessments. Extrapolating from human risks remains hypothetical. Conclusions: useful toxicity, but better needed today's context regulation evaluation claims. (Cancer Epidemiol Biomarkers Prev 2009;18(12):3263–304)