Distribution of microtubules and microfilaments in thyroid follicular epithelial cells of normal, TSH-treated, aged, and hypophysectomized rats.
作者： H. Kurihara , K. Uchida , H. Fujita
DOI: 10.1007/BF00315848
关键词: Biology 、 Cytoplasm 、 Pseudopodia 、 Epithelial polarity 、 Cell biology 、 Thyroid 、 Endocrinology 、 Tubulin 、 Internal medicine 、 Microfilament 、 Cytoskeleton 、 Microtubule
摘要: We investigated the distribution of microtubules and microfilaments in rat thyroid follicular epithelial cells by applying an immunofluorescence technique with monoclonal antibodies against tubulin staining sections rhodamine-phalloidin. In normal cells, run longitudinally from apical region to basal intersecting each other. addition, intense labelling was observed part cell. The ultrastructural examinations showed that often along plasma membrane. Dot-like anti-tubulin perinuclear space, but not were recognized nucleus. Microfilaments bound rhodamine-phalloidin distributed mainly beneath membrane, portion basolateral membrane scarcely positive. pole follicle cell also decorated anti-microtubule-associated protein-2 (MAP-2). After TSH stimulation, intensity immunocytochemical remarkably increased cytoplasm. Simultaneously, at region, increased. Microtubules appeared pseudopods after injection. hypophysectomized or aged rats, decreased height, both immunofluorescent markedly decreased. These results indicate polymerization vary functional stage.
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sci-hub.se 参考文章(42)
J Davis, V Bennett, Microtubule-associated protein 2, a microtubule-associated protein from brain, is immunologically related to the alpha subunit of erythrocyte spectrin. Journal of Biological Chemistry. ,vol. 257, pp. 5816- 5820 ,(1982) , 10.1016/S0021-9258(19)83851-6
J. WOLFF, J.A. WILLIAMS, The Role of Microtubules and Microfilaments in Thyroid Secretion Recent Progress in Hormone Research. ,vol. 29, pp. 229- 285 ,(1973) , 10.1016/B978-0-12-571129-6.50010-5
G. Wiche, E. Briones, C. Koszka, U. Artlieb, R. Krepler, Widespread occurrence of polypeptides related to neurotubule-associated proteins (MAP-1 and MAP-2) in non-neuronal cells and tissues. The EMBO Journal. ,vol. 3, pp. 991- 998 ,(1984) , 10.1002/J.1460-2075.1984.TB01918.X
Hisao Fujita, Fine Structure of the Thyroid Gland International Review of Cytology. ,vol. 40, pp. 197- 280 ,(1975) , 10.1016/S0074-7696(08)60954-7
W E Theurkauf, R B Vallee, Molecular characterization of the cAMP-dependent protein kinase bound to microtubule-associated protein 2. Journal of Biological Chemistry. ,vol. 257, pp. 3284- 3290 ,(1982) , 10.1016/S0021-9258(19)81107-9
J.E. Dumont, The action of thyrotropin on thyroid metabolism. Vitamins and Hormones Series. ,vol. 29, pp. 287- 412 ,(1971) , 10.1016/S0083-6729(08)60051-5
Rolf Seljelid, Endocytosis in thyroid follicle cells: II. A microinjection study of the origin of colloid droplets Journal of Ultrastructure Research. ,vol. 17, pp. 401- 420 ,(1967) , 10.1016/S0022-5320(67)80132-1
J. A. Williams, J. Wolff, Possible Role of Microtubules in Thyroid Secretion Proceedings of the National Academy of Sciences of the United States of America. ,vol. 67, pp. 1901- 1908 ,(1970) , 10.1073/PNAS.67.4.1901
P NEVE, C WILLEMS, J DUMONT, Involvement of the microtubule-microfilament system in thyroid secretion. Experimental Cell Research. ,vol. 63, pp. 457- 460 ,(1970) , 10.1016/0014-4827(70)90238-7
R. EKHOLM, L. E. ERICSON, J.-O. JOSEFSSON, A. MELANDER, In Vivo Action of Vinblastine on Thyroid Ultrastructure and Hormone Secretion Endocrinology. ,vol. 94, pp. 641- 649 ,(1974) , 10.1210/ENDO-94-3-641