Microgravity induces prostaglandin E2 and interleukin-6 production in normal rat osteoblasts: role in bone demineralization.

摘要: Abstract It has been suggested that microgravity alters bone metabolism. Evidence for this phenomenon includes the negative calcium balance and decreased density in astronauts, as well as, inhibition of formation rats flown 2 to 3 weeks. However, specific mechanisms modulate these changes are unknown. The purpose study was clarify mechanism microgravity-induced demineralization using normal rat osteoblasts obtained from femur marrow cultures. were cultured 5 days during a Shuttle-Spacelab flight (STS-65). After collection culture medium, cellular DNA RNA fixed on board. Enzyme-immunoassay medium prostaglandin E2 (PGE2) indicated induced 4.5-to 136-fold increase samples compared ground control This PGE2 production consistent with 3.3-to 9.5-fold elevation inducible G/H synthase-2 (PGHS-2) mRNA, quantitated by reverse transcription-polymerase chain reaction (RT-PCR). mRNA induction constitutive isozyme PGHS-1 less than PGHS-2. interleukin-6 (IL-6) also increased (6.4-to 9.3-fold) controls. Since IL-6 both known play role osteoclast resorption, data provide molecular contribute our understanding alterations resorption process.