Degradation of brain natriuretic peptide by neutral endopeptidase: species specific sites of proteolysis determined by mass spectrometry.
作者： Jon A. Norman , Deborah Little , Mark Bolgar , Gerald Di Donato
DOI: 10.1016/S0006-291X(05)81194-5
关键词: Cleavage (embryo) 、 Brain natriuretic peptide 、 Stereochemistry 、 Proteolysis 、 Chemistry 、 Biochemistry 、 Fast atom bombardment 、 Mass spectrometry 、 Neprilysin 、 Enzyme 、 Endopeptidase
摘要: Brain natriuretic peptide (BNP) from 3 different species was cleaved by neutral endopeptidase (NEP) and the products separated HPLC. The newly formed were identified fast atom bombardment or nebulizer-assisted electrospray mass spectrometry to elucidate sites of proteolysis. Porcine BNP at Arg8-Leu9 Ser14-Leu15 bonds. Rat Arg23-Leu24 Arg30-Leu31 Human Pro2-Lys3, Met4-Val5 Arg17-Leu18 Cys-Phe bond which is present in all not NEP.
elsevier.com
sci-hub.se 参考文章(19)
J A Koehn, J A Norman, B N Jones, L LeSueur, Y Sakane, R D Ghai, Degradation of atrial natriuretic factor by kidney cortex membranes. Isolation and characterization of the primary proteolytic product. Journal of Biological Chemistry. ,vol. 262, pp. 11623- 11627 ,(1987) , 10.1016/S0021-9258(18)60854-3
Richard D. Smith, Joseph A. Loo, Charles G. Edmonds, Charles J. Barinaga, Harold R. Udseth, New developments in biochemical mass spectrometry: electrospray ionization Analytical Chemistry. ,vol. 62, pp. 882- 899 ,(1990) , 10.1021/AC00208A002
Masashi Mukoyama, Kazuwa Nakao, Yoshihiko Saito, Yoshihiro Ogawa, Kiminori Hosoda, Shin-Ichi Suga, Gotaro Shirakami, Michihisa Jougasaki, Hiroo Imura, Human brain natriuretic peptide, a novel cardiac hormone The Lancet. ,vol. 335, pp. 801- 802 ,(1990) , 10.1016/0140-6736(90)90925-U
Tetsuji Sudoh, Kenji Kangawa, Naoto Minamino, Hisayuki Matsuo, A new natriuretic peptide in porcine brain Nature. ,vol. 332, pp. 78- 81 ,(1988) , 10.1038/332078A0
Andries P. Bruins, Thomas R. Covey, Jack D. Henion, Ion spray interface for combined liquid chromatography/atmospheric pressure ionization mass spectrometry Analytical Chemistry. ,vol. 59, pp. 2642- 2646 ,(1987) , 10.1021/AC00149A003
Jean-Charles Schwartz, Claude Gros, Jeanne-Marie Lecomte, Jean Bralet, Enkephalinase (EC 3.4.24.11) inhibitors: Protection of endogenous ANF against inactivation and potential therapeutic applications Life Sciences. ,vol. 47, pp. 1279- 1297 ,(1990) , 10.1016/0024-3205(90)90192-T
Craig M. Whitehouse, Robert N. Dreyer, Masamichi. Yamashita, John B. Fenn, Electrospray interface for liquid chromatographs and mass spectrometers Analytical Chemistry. ,vol. 57, pp. 675- 679 ,(1985) , 10.1021/AC00280A023
Masahito Aburaya, Naoto Minamino, Kenji Kangawa, Kenjiro Tanaka, Hisayuki Matsuo, Distribution and molecular forms of brain natriuretic peptide in porcine heart and blood. Biochemical and Biophysical Research Communications. ,vol. 165, pp. 872- 879 ,(1989) , 10.1016/S0006-291X(89)80047-6
Tim Yandle, Ian Crozier, Gary Nicholls, Eric Espiner, Alan Carne, Stephen Brennan, Amino acid sequence of atrial natriuretic peptides in human coronary sinus plasma Biochemical and Biophysical Research Communications. ,vol. 146, pp. 832- 839 ,(1987) , 10.1016/0006-291X(87)90606-1
Ming-shi Chang, David G. Lowe, Martyn Lewis, Renate Hellmiss, Ellson Chen, David V. Goeddel, Differential activation by atrial and brain natriuretic peptides of two different receptor guanylate cyclases Nature. ,vol. 341, pp. 68- 72 ,(1989) , 10.1038/341068A0