Bioluminescence assay of calcium with a liquid scintillation counter

作者: C. Oveby , R. Kant , S. Batra

DOI: 10.1007/BF01990443

关键词: CalciumLiquid scintillation countingBioluminescenceAequorinScintillation counterChemistryLuminescenceAnalytical chemistryTissue sampleGeneral Biochemistry, Genetics and Molecular BiologyMolecular medicineCell biologyMolecular biologyPharmacologyCellular and Molecular Neuroscience

摘要: The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. concentration total and free Ca-EGTA buffers could be determined measuring the decay liquid scintillation counter. method is also suitable for determining small tissue samples.

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