Development and characterization of simple sequence repeat markers for genetic analyses of Sargassum horneri (Sargassaceae, Phaeophyta) populations in Kyoto, Japan
作者： Nakao Kubo , Akio Douke , Tomokazu Nishigaki , Gento Tsuji
DOI: 10.1007/S10811-016-1041-Y
关键词: Locus (genetics) 、 Sargassum horneri 、 Genetics 、 Genetic diversity 、 Microsatellite 、 Sargassum 、 Genetic marker 、 Genotyping 、 Biology 、 Sargassaceae
摘要: Simple sequence repeat (SSR) markers were developed for the brown alga Sargassum horneri (also known as “akamoku”) from an SSR-enriched genomic library. Of 22 SSR developed, 10 primer pairs produced clearly distinguishable DNA bands, indicating polymorphisms in S. populations collected around Wakasa Bay, Kyoto, Japan. Ten among newly SSRs also applicable to any of five other species tested. To assess utility classification horneri, we genotyped 86 akamoku individuals six (Naryu, Obase, Oshima, Satohami, Tai, and Taiza). We detected 148 alleles using 11 (10 new 1 previously published). The allele number per locus ranged 4 32 (average, 13.5). Based on genotyping data markers, three separated a phylogram. These results indicate that this study are informative useful genetic analyses species.
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