Synergistic activity between colistin and the ionic liquids 1-methyl-3-dodecylimidazolium bromide, 1-dodecyl-1-methylpyrrolidinium bromide, or 1-dodecyl-1-methylpiperidinium bromide against Gram-negative bacteria.

摘要: Abstract Objectives Ionic liquids have shown potential for applications as antimicrobials. Their antimicrobial activity has been to be higher against Gram-positive than Gram-negative bacteria, suggesting a protective role the outer membrane of microorganisms. Colistin is last-resort antibiotic often used treating infections sustained by multi-drug resistant bacteria. interacts with bacterial lipopolysaccharide, thus altering structure and increasing permeability membrane. The aim this study was investigate interaction between colistin ionic 1-methyl-3-dodecylimidazolium bromide, 1-dodecyl-1-methylpyrrolidinium 1-dodecyl-1-methylpiperidinium bromide bacteria clinical importance such asEscherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii. Methods evaluated checkerboard assay. Bacterial killing assays againstP. aeruginosa were carried out assess whether synergistic combinations bactericidal. Results results showed that all three interacted synergistically againstK. P. A. baumannii but not Escherichia which resulted more sensitive compared other tested species. no hemolytic activity. effect each one Conclusion Overall, obtained suggest might in combination possible combat