Characterisation of potential regulators of PAMP-triggered immunity

摘要: An essential part of plant innate immunity is the perception pathogen-associated molecular patterns (PAMPs) through surface-localised pattern-recognition receptors (PRRs). EFR and FLS2 are well characterised PRRs, which recognise bacterial elongation factor Tu flagellin, respectively. A variety downstream responses upon PAMP have been described. However, it still poorly understood how EFR- FLS2-dependent signalling mediated. Here, I used two different approaches in order to identify novel components both pathways. First, seven candidate genes a predicted flagellin-dependent gene expression network, regulate each other’s manner, therefore, potentially involved pathway. Further characterisation revealed that mutation at least three was affecting flg22-mediated signalling. In addition, protein phosphatase 2C (PP2C), had identified yeast two-hybrid screen with cytoplasmic domain therefore referred as PIE (PP2C-interacting EFR). Using Co-IP experiments, confirmed associates planta. Furthermore, also BIK1, co-regulator Interestingly, dissociates from complexes perception. expressed planta E. coli exhibits activity we showed dephosphorylates EFR, BIK1 vitro. As expected bio-informatic predictions, phosphorylated demonstrated kinase activities partially required for phosphorylation. Both overexpression pie knock-down lines display reduced PAMP-triggered responses, indicating an optimal level proper induction signalling. All together, these results imply regulator