Salt treatment induces frost hardiness in leaves and isolated thylakoids from spinach.
作者： J. E. Schmidt , J. M. Schmitt , W. M. Kaiser , D. K. Hincha
DOI: 10.1007/BF00407008
关键词: Horticulture 、 Frost (temperature) 、 Chenopodiaceae 、 Spinacia 、 Chloroplast 、 Spinach 、 Botany 、 Dehydration 、 Thylakoid 、 Chlorophyll fluorescence 、 Biology
摘要: Frost hardiness of spinach (Spinacia oleracea L.) leaves was increased by high concentrations NaCl in the hydroponic culture medium. Freezing damage determined measurement slow chlorophyll fluorescence quenching after freezing leaves. Both osmolality leaf sap and forst were linearly correlated with salt concentration occurred, irrespective extent frost hardening, when dehydration cells during extracellular ice formation decreased cellular volume to approximately 14% unfrozen cells. The resistance isolated, washed thylakoids against mechanical chemical investigated. Chemical caused accumulation measured as release chloroplast coupling factor (CF1; EC 3.6.1.3), lumenal protein plastocyanin from membranes an in-vitro freeze-thaw cycle. Isolated salt-treated frost-hardy those plants hardened under natural conditions did not exhibit improved tolerance stress exerted concentrations. They were, however, more hardy than unhardened control freezing.
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