A glyceraldehyde-3-phosphate dehydrogenase pseudogene on the short arm of the human X chromosomes defines a multigene family.

摘要: A human X chromosome-derived gene sequence which recognises an abundant, 1.2-kb mRNA in several cell types was previously isolated during a study to identify expressed sequences from chromosome recombinant library. Further characterisation of this clone, acronym OA1, has shown that it maps the short arm X, at Xp21 Xp22. 777-bp fragment clone hybridizes been sequenced, and inferred amino acid shows 80% homology with published protein for muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The even higher (87%) pig GAPDH. OA1 selects translates vitro into polypeptide 36 K, subunit size However, X-sequence is most probably pseudogene whose structure consistent having arisen by reverse transcription GAPDH or GAPDH-related followed insertion chromosome. portion DNA fragments mouse DNA, six fibroblast cDNA clones cross-hybridize same genomic as OA1. This series identifies new, conserved multigene family.