β-Carotene and Inhibitors of Iron Absorption Modify Iron Uptake by Caco-2 Cells

摘要: A National fortification program instituted in Venezuela 1993 reduced iron deficiency and anemia by half only 1 y. The mixture contained ferrous fumarate, vitamin other vitamins. We conducted experiments to characterize fumarate uptake Caco-2 cells. Increasing amounts of A, phytate, tannic acid beta-carotene were added incubation mixtures using a range concentrations that included the molar ratios used Venezuelan program. Cells incubated for h at 37 degrees C with kBq (59)Fe compound be evaluated. They then rinsed, trypsinized counted measure uptake. Effects ascorbic acid, days culture use flasks or inserts also Optimal conditions pH 5.5, presence 16 d culture. Use did not affect Vitamin significantly increase under experimental employed. However, (6 micromol/L) increased compared no addition (114.9 +/- 6.3 47.2 5.9 pmol/mg cell protein, respectively). Moreover, phytates generally overcame inhibitory effects both compounds depending on their concentrations. conclude improves overcomes inhibition potent inhibitors absorption. These validated usefulness model system evaluate metabolism.