Seasonal prevalence of serum antibodies to whole cell and recombinant antigens of Borrelia burgdorferi and Anaplasma phagocytophilum in white-tailed deer in Connecticut.

摘要: Whole-blood samples were obtained from 214 white-tailed deer (Odocoileus virginianus) representing 44 sites in Connecticut (USA) during 1992, 1993, 1996, 1999, and 2000 through 2006. Sera analyzed for total antibodies to whole-cell or recombinant antigens of Borrelia burgdorferi sensu stricto Anaplasma phagocytophilum, the respective causative agents Lyme borreliosis human granulocytic anaplasmosis. Deer sera contained both bacteria different seasons throughout 11-yr study. Of 224 tested, 141 (63%) B. a polyvalent enzyme-linked immunosorbent assay, whereas 124 (55%) positive A. phagocytophilum by indirect fluorescent antibody staining. Use highly specific (VlsE protein phagocytophilum) provided strong confirmatory results past current infections. There was coexistence 72 (32%) sera. Analyses 18 eight that marked, released, recaptured, showed minimal changes titer over sampling time intervals ranging 17 days 5.1 yr. Relatively high prevalences bacterial years reaffirm there are well-established foci tick-borne infections probably reflect frequent exposure infected Ixodes scapularis ticks. November December is suitable period obtain blood conduct serosurveillance bacteria.