RAPD-PCR of Lichens

作者: H. Thorsten Lumbsch , Imke Schmitt

DOI: 10.1007/978-3-642-56359-1_25

关键词: GeneticsGenetic variationBiologyPolymerase chain reactionDNALichenPhylogenetic treeRAPDInterspecific competitionHomology (biology)

摘要: Randomly amplified polymorphic DNA (RAPD)- polymerase chain reaction (PCR) is a simple and inexpensive tool enabling the study of genetic variation at population level. It can also be applied species or subgeneric level to examine phylogenetic questions (e.g., Yoon Bae 1995, Altomare et al 1997, Gandeboeuf al. 1997). However, (1996) showed that considerable fraction (13%) RAPD fragments in interspecific comparisons are not homologous. The homology problem will less important when very closely related organisms studied, e.g. an infraspecific

参考文章(20)
Electrophoresis of Nucleic Acids Blackwell Publishing Ltd.. pp. 126- 127 ,(2009) , 10.1002/9781444313611.CH47
Vernon Ahmadjian, The lichen symbiosis ,(1967)
A. BURT, D. A. CARTER, T. J. WHITE, J. W. TAYLOR, DNA sequencing with arbitrary primer pairs Molecular Ecology. ,vol. 3, pp. 523- 525 ,(1994) , 10.1111/J.1365-294X.1994.TB00131.X
M. Grube, P.T. Depriest, A. Gargas, J. Hafellner, DNA isolation from lichen ascomata Fungal Biology. ,vol. 99, pp. 1321- 1324 ,(1995) , 10.1016/S0953-7562(09)81215-X
Masatoshi Nei, Wen-Hsiung Li, Mathematical model for studying genetic variation in terms of restriction endonucleases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 5269- 5273 ,(1979) , 10.1073/PNAS.76.10.5269
L. H. RIESEBERG, Homology among RAPD fragments in interspecific comparisons Molecular Ecology. ,vol. 5, pp. 99- 105 ,(1996) , 10.1111/J.1365-294X.1996.TB00295.X
Claudio Altomare, Antonio Logrieco, Orlando Petrini, Antonio Bottalico, Taxonomic relationships among the toxigenic species Fusarium acuminatum, Fusarium sporotrichioides and Fusarium tricinctum by isozyme analysis and RAPD assay Botany. ,vol. 75, pp. 1674- 1684 ,(1997) , 10.1139/B97-881
Katileena Lohtander, Mari Källersjö, Anders Tehler, DISPERSAL STRATEGIES INROCCELLINA CAPENSIS(ARTHONIALES) Lichenologist. ,vol. 30, pp. 341- 350 ,(1998) , 10.1006/LICH.1998.0154