作者: H. Thorsten Lumbsch , Imke Schmitt
DOI: 10.1007/978-3-642-56359-1_25
关键词: Genetics 、 Genetic variation 、 Biology 、 Polymerase chain reaction 、 DNA 、 Lichen 、 Phylogenetic tree 、 RAPD 、 Interspecific competition 、 Homology (biology)
摘要: Randomly amplified polymorphic DNA (RAPD)- polymerase chain reaction (PCR) is a simple and inexpensive tool enabling the study of genetic variation at population level. It can also be applied species or subgeneric level to examine phylogenetic questions (e.g., Yoon Bae 1995, Altomare et al 1997, Gandeboeuf al. 1997). However, (1996) showed that considerable fraction (13%) RAPD fragments in interspecific comparisons are not homologous. The homology problem will less important when very closely related organisms studied, e.g. an infraspecific