Sedimentary DNA Reveals Cyanobacterial Community Diversity over 200 Years in Two Perialpine Lakes.

摘要: ABSTRACT We reconstructed cyanobacterial community structure and phylogeny using DNA that was isolated from layers of stratified sediments spanning 200 years lake history in the perialpine lakes Greifensee Lake Zurich (Switzerland). Community analysis based on amplification sequencing a 400-nucleotide (nt)-long 16S rRNA fragment specific to Cyanobacteria revealed operational taxonomic units (OTUs) capturing whole phylum, including representatives newly characterized clade termed Melainabacteria, which shares common ancestry with has not been previously described lakes. The reconstruction richness phylogenetic validated data set consisting 40 pelagic microscopic counts each lake. identified OTUs assigned taxa known be present found strong significant relationship (adjusted R2 = 0.89; P IMPORTANCE Cyanobacterial blooms can produce toxins affect water quality, especially under eutrophic conditions, are consequence human-induced climate warming increased nutrient availability. Lakes worldwide have suffered regular over last century. lack long-term limits our understanding how these form. successfully past diversity communities two hundred by genes preserved Switzerland. changes time results existing collected same years. This work shows potential approach for addressing important ecological questions about effects changing environment ecology.