Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications
作者： Yehonatan Ravenna , Lin Xia , Jenny Gun , Alexey A. Mikhaylov , Alexander G. Medvedev
DOI: 10.1021/ACS.ANALCHEM.5B02949
关键词: Electron transfer 、 Inorganic chemistry 、 Graphene 、 Electrode 、 Chemistry 、 Glucose dehydrogenase 、 Oxide 、 Redox 、 Biosensor 、 Flavin adenine dinucleotide
摘要: A novel composite material for the encapsulation of redox enzymes was prepared. Reduced graphene oxide film with adsorbed phenothiazone used as a highly efficient electron transfer between flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase and electrodes. Measured potential oxidation lower than 0 V vs Ag/AgCl electrode. The fabricated biosensor showed high sensitivity 42 mA M(-1) cm(-2), linear range detection 0.5-12 mM, good reproducibility stability well selectivity different interfering compounds. In semibiofuel cell configuration, hybrid generated power output 345 μW cm(-2). These results demonstrate promising this composition in various bioelectronic applications.
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