Aptamer affinity chromatography:: combinatorial chemistry applied to protein purification
作者： Timothy S Romig , Carol Bell , Daniel W Drolet
DOI: 10.1016/S0378-4347(99)00243-1
关键词: Chemistry 、 Fusion protein 、 Chromatography 、 Oligonucleotide 、 Combinatorial chemistry 、 Systematic evolution of ligands by exponential enrichment 、 Elution 、 Tandem affinity purification 、 Protein purification 、 Affinity chromatography 、 Aptamer
摘要: … (L), flow through (F/T) and EDTA elution (E) fractions respectively. Lanes 5–7 contain the column load, flow through … Lanes 9–11 contain the column load, flow through and NaCl elution …
elsevier.com
sci-hub.se 参考文章(42)
ED Harlow, David Lane, A Laboratory manual Cold Spring Harbor Laboratory. ,(1989)
Doug Irvine, Craig Tuerk, Larry Gold, SELEXION. Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis. Journal of Molecular Biology. ,vol. 222, pp. 739- 761 ,(1991) , 10.1016/0022-2836(91)90509-5
S C Dreskin, T W Wiegand, P B Williams, J P Kinet, D Tasset, M H Jouvin, High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. Journal of Immunology. ,vol. 157, pp. 221- 230 ,(1996)
GS Kansas, Selectins and their ligands: current concepts and controversies. Blood. ,vol. 88, pp. 3259- 3287 ,(1996) , 10.1182/BLOOD.V88.9.3259.BLOODJOURNAL8893259
C Chen, H Okayama, High-efficiency transformation of mammalian cells by plasmid DNA. Molecular and Cellular Biology. ,vol. 7, pp. 2745- 2752 ,(1987) , 10.1128/MCB.7.8.2745
Josephine Charlton, Gary P. Kirschenheuter, Drew Smith, Highly potent irreversible inhibitors of neutrophil elastase generated by selection from a randomized DNA-valine phosphonate library. Biochemistry. ,vol. 36, pp. 3018- 3026 ,(1997) , 10.1021/BI962669H
Gregory J. Connell, Mali Illangesekare, Michael Yarus, Three small ribooligonucleotides with specific arginine sites Biochemistry. ,vol. 32, pp. 5497- 5502 ,(1993) , 10.1021/BI00072A002
C. Tuerk, S. MacDougal, L. Gold, RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 6988- 6992 ,(1992) , 10.1073/PNAS.89.15.6988
John Maclennan, Engineering Microprotein Ligands for Large–Scale Affinity Purification Nature Biotechnology. ,vol. 13, pp. 1180- 1183 ,(1995) , 10.1038/NBT1195-1180
Richard D. Cummings, David F. Smith, The selectin family of carbohydrate-binding proteins: Structure and importance of carbohydrate ligands for cell adhesion BioEssays. ,vol. 14, pp. 849- 856 ,(1992) , 10.1002/BIES.950141210