Kiwi fruit InDel molecular marker and screening method and application thereof

摘要: The invention discloses a kiwi fruit InDel molecular marker and screening method application thereof. Two different cold-proof Actinidia arguta genome DNAs are used as research subjects, resequencing of four variety genes is carried out, primers designed according to data, PCR (polymerase chain reaction) amplification, agarose electrophoresis non-denaturing polyacrylamide gel out; polymorphism detection in screen out 14 pairs primers, the applicable genetic diversity analysis for chinensis, deliciosa like also researches, such hybrid offspring authenticity identification, map construction, molecule-assisted breeding. embodiment has good mutation stability low difficulty, allow insertion/loss large fragments, allows analysis, with steps that may be simplified.